(725) 287-7791
Donate|Patient Portal|Staff Login
Research Program

CRISPR Protein Engineering
Pipeline for Students

Free access to our GPU-accelerated computational protein engineering platform. Design Cas protein variants, score mutations with ESM-2, generate de novo binders with BoltzGen, and search protein databases with vector similarity -- all through a simple REST API.

Request Student AccessAPI Documentation

What You Can Do

A complete computational toolkit for CRISPR protein engineering research, powered by state-of-the-art deep learning models.

ESM-2 Protein Language Model

Access the 2.8 billion parameter ESM-2 model for mutation scoring via masked marginal likelihood, protein embeddings, and evolution library design.

BoltzGen Binder Design

Design de novo protein binders with confidence scoring including iPAE and iPTM metrics for your CRISPR targets.

pgvector Similarity Search

Store and search protein sequences using 2560-dimensional ESM-2 embeddings with PostgreSQL vector similarity.

Variant Engineering

Generate mutation libraries targeting specific residues with log-likelihood scoring to identify high-potential Cas protein variants.

Structure Prediction

On-demand ESMFold 3D structure prediction for top-scoring variants to validate computational designs.

GPU-Accelerated Pipeline

All computations run on dedicated NVIDIA GPUs. No local hardware required -- just use the REST API from any device.

Get Started in 4 Steps

01

Request Access

Submit your .edu email and research purpose. Access is granted within 24 hours for verified students.

02

Get API Credentials

Receive your API key and endpoint URL. Full Swagger documentation is available at /docs.

03

Add Your Proteins

Register parent Cas proteins with their sequences. The system generates ESM-2 embeddings automatically.

04

Engineer Variants

Design mutation libraries, score substitutions, predict structures, and design binders -- all via API.

API Reference

All endpoints accept and return JSON. Full interactive documentation available at the Swagger UI.

https://crispr-api.helixbiomedical.us
POST/proteins
POST/engineer
POST/mutations/score
POST/binders/design
POST/search
POST/structures/predict
GET/stats
POST/embeddings/generate
RequestPOST /engineer
curl -X POST /engineer \
  -H "Authorization: Bearer YOUR_API_KEY" \
  -H "Content-Type: application/json" \
  -d '{
    "parent_name": "OpenCRISPR-1",
    "library_size": 96,
    "focus_regions": [
      {"start": 762, "end": 780}
    ],
    "max_mutations": 3,
    "predict_structures": false
  }'
Response200 OK
{
  "job_id": "eng_abc123",
  "parent": "OpenCRISPR-1",
  "variants_designed": 96,
  "top_variant": {
    "name": "OpenCRISPR-1_V3",
    "mutations": ["A762G", "T770S", "K778R"],
    "score": 4.82,
    "improvement": "+23% vs wild-type"
  },
  "status": "completed"
}

Who Can Access This?

Undergraduate and graduate students in biology, biochemistry, or bioinformatics

Postdoctoral researchers and faculty at accredited institutions

Independent researchers with published work in protein engineering

High school students in advanced biology programs (with faculty sponsor)

Non-Commercial Use Only

This resource is provided free of charge for academic and educational purposes. Commercial use requires a separate license agreement.

Ready to Start Engineering?

Submit your access request with your .edu email address and a brief description of your research. Most requests are approved within 24 hours.

Request Access Now